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Identification of cultured Brachionus rotifers based on RFLP and SSCP screening
Papakostas, S.; Dooms, S.; Christodoulou, M.; Triantafyllidis, A.; Kappas, I.; Dierckens, K.; Bossier, P.; Sorgeloos, P.; Abatzopoulos, T.J. (2006). Identification of cultured Brachionus rotifers based on RFLP and SSCP screening. Mar. Biotechnol. 8(5): 547-559. dx.doi.org/10.1007/s10126-005-6181-z
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236
Peer reviewed article  
Available in  Authors 
    Vlaams Instituut voor de Zee: Open Marine Archive 115402 [ download pdf ]
Keywords
    Acids > Organic compounds > Organic acids > Nucleic acids > DNA
    Aquaculture facilities > Hatcheries
    Cell constituents > Cell organelles > Mitochondria
    Rotifera [WoRMS]
    Marine/Coastal
Author keywords
    COI restriction map; hatchery strains; rotifer clones; mitochondrial DNA; 16S rRNA
Authors  Top 
  • Papakostas, S.
  • Dooms, S.
  • Christodoulou, M.
  • Triantafyllidis, A.
  • Kappas, I.
  • Dierckens, K.
  • Bossier, P.
  • Sorgeloos, P.
  • Abatzopoulos, T.J.
Abstract
    The marine finfish industry worldwide depends greatly on the mass culture of Brachionus rotifers. Recently, molecular data have revealed a more complicated view about the species status of Brachionus rotifers than previous mainly morphological assessments. Under this view, Brachionus rotifers are comprised of many morphologically similar, albeit genetically differentiated, cryptic members of larger groups. A redefinition of the cultured rotifer species/biotypes is therefore needed if aquaculture is to reach higher levels of standardization and predictability. In this work, restriction fragment length polymorphism (RFLP) and single-strand conformational polymorphism (SSCP) methods are applied to the COI and 16S rRNA mitochondrial genes. A detailed COI restriction map was constructed, using sequence data from all known representatives of Brachionus phylogroups. Therefore, it is the first time that such an extended restriction database has been produced. Several restriction endonucleases are proposed for the discrimination of the different Brachionus species/biotypes. Furthermore, eight different SSCP gel alleles are described for the 16S region. Using these data, five Brachionus species/biotypes were identified in 78 samples collected from laboratories and hatcheries around the world.
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